趋化因子C-X-C-基元配体15(CXCL15)重组蛋白说明书-分析方法-资讯-生物在线

趋化因子C-X-C-基元配体15(CXCL15)重组蛋白说明书

作者:上海沪震实业有限公司 2023-04-25T00:00 (访问量:933)

 趋化因子C-X-C-基元配体15(CXCL15)重组蛋白说明书

Recombinant Chemokine C-X-C-Motif Ligand 15 (CXCL15)

[PROPERTIES]

 

Source: Prokaryotic expression

Host: E.coli

Residues: Gln26~Ala167

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 150µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.2

Predicted Molecular Mass: 18.6kDa

Accurate Molecular Mass: 24kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[SEQUENCE]

2022121711442946212

[IDENTIFICATION]

2022121711443402256

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用
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